EHEC genomes – plasmid

A brief look at the genes present in the outbreak strains and not in the reference strain Ec55989 reveals a large number of contigs with similarity to the plasmid pEC_Bactec (by NCBI blast)… a quick MUMmer alignment and ACT visualisation confirms that an IncI plasmid similar to pEC_bactec (GU371927.1, ref here) is present, including the beta-lactamase gene CTX-M-15 adjacent to ISEcp1 (MIRA assembly of TY2482, contig husec41_c30). In this image, the TW2482 assembly is on top, pEC_Bactec is on bottom.

MIRA assembly of TW2482 vs pEC_Bactec

Update: Note this is also similar to pEK204, which was isolated from UPEC and we have recently found very similar plasmids (also carrying blaCTX-M-15) circulating in Shigella in Vietnam and Klebsiella pneumoniae in Australia. So this would seem to be spreading around the Enterobacteriaceae.

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6 thoughts on “EHEC genomes – plasmid

  1. Pingback: My contribution to the ‘HUSEC41-strains-are-not-that-new’ debate « The Alignment Gap

  2. Pingback: Some updates on the crowd-sourcing around HUSEC41 genome analyses « The Alignment Gap

  3. Pingback: EHEC genomes – CTX-M-15 and Shigella

  4. Pingback: EHEC genomes – CTX-M-15 and Shigella

  5. Pingback: Escherichia coli plasmid pEC_Bactec is a part of EHEC H112180280 strain | the oh no sequences! blog

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